mabtech elispot reader  (Mabtech Inc)

Journal: iScience

Article Title: Jchain -diphtheria toxin receptor mice allow for depletion of antibody-secreting cells and analysis of differentiation kinetics

doi: 10.1016/j.isci.2025.113946

Figure Lengend Snippet: ASCs develop normally in the spleen, bone marrow and thymus of J-DTR mice (A–C) Total ASC numbers for (A) spleen, (B) bone marrow, and (C) thymus of WT and J-DTR mice. Data presented on log 10 scale. (D) Representative ELISpot images showing IgM, IgG, and IgA spot formation from spleen, bone marrow, and thymus of WT and J-DTR mice. 10 5 cells per well were plated in triplicate. Numbers indicate spots counted per well following background subtraction of identical wells not coated with capture Ab. (E–G) Numbers of (E) IgM, (F) IgG, and (G) IgA spots per 10 5 cells from spleen, bone marrow and thymus of WT and J-DTR mice. (H–J) Plasma concentration of (H) IgM, (I) IgG, and (J) IgA from WT and J-DTR mice. Data presented on log 10 scale. (K) Fecal IgA concentration from WT and J-DTR mice. Data presented on log 10 scale. (A–C) Symbols represent individual 3–7 months old female (orange) and male (blue) mice. Data pooled from 9 independent experiments. Horizontal lines represent mean ± SEM. WT: female n = 8, male n = 10; J-DTR: female n = 10, male n = 11. Statistics: Unpaired Student’s t test between genotypes. (E–G) Symbols represent individual wells plated in triplicate from 3 to 5 months old female (orange) and male (blue) WT and J-DTR mice. Values shown represent data following subtraction of spots detected in wells that were not coated with capture Ab (background). Data pooled from 3 independent experiments. Horizontal lines represent mean ± SEM. WT: female n = 4, male n = 5. J-DTR: female n = 5, male n = 6. Statistics: Unpaired Student’s t test between genotypes. (H–K) Symbols represent individual 3–5 months old female (orange) and male (blue) WT and J-DTR mice. Data pooled from 3 to 4 independent experiments. Horizontal lines represent mean ± SEM. WT: female n = 4, male n = 5. J-DTR: female n = 5, male n = 6. Statistics: Unpaired Student’s t test between genotypes.

Article Snippet: Well backings were removed, and plates dried at RT O/N after which “spots” were visualized with a Mabtech ASTOR ELISPOT reader.

Techniques: Enzyme-linked Immunospot, Clinical Proteomics, Concentration Assay

Journal: iScience

Article Title: Jchain -diphtheria toxin receptor mice allow for depletion of antibody-secreting cells and analysis of differentiation kinetics

doi: 10.1016/j.isci.2025.113946

Figure Lengend Snippet: Single dose administration of DT leads to the loss of Ab spot formation and fecal IgA in J-DTR mice (A) Representative ELISpot images showing IgM, IgG, and IgA spot formation from spleen of WT and J-DTR mice treated with PBS or DT. 10 5 cells per well were plated in triplicate. Numbers indicate spots counted per well following background subtraction of identical wells not coated with capture Ab. (B–D) Numbers of IgM spots per 10 5 cells from (B) spleen, (C) bone marrow, and (D) thymus of WT and J-DTR mice treated with PBS or DT. (E–G) Numbers of IgG spots per 10 5 cells from (E) spleen, (F) bone marrow, and (G) thymus of WT and J-DTR mice treated with PBS or DT. (H–J) Numbers of IgA spots per 10 5 cells from (H) spleen, (I) bone marrow, and (J) thymus of WT and J-DTR mice treated with PBS or DT. (K) Fecal IgA concentration from WT and J-DTR mice treated with PBS or DT. Data presented on log 10 scale. (B–J) Symbols represent individual wells plated in triplicate from 3 to 5 months old female (orange) and male (blue) WT and J-DTR mice. Values shown represent data following subtraction of spots detected in wells that were not coated with capture Ab (background). Some symbols overlap due to having the same value (e.g., 0). Data pooled from 3 independent experiments. Horizontal lines represent mean ± SEM. WT PBS: female n = 1, male n = 2; WT DT: female n = 2, male n = 1; J-DTR PBS: female n = 2, male n = 2; J-DTR DT: female n = 1, male n = 3. Statistics: Unpaired Student’s t test with comparisons made between PBS and DT treatments within a genotype. (K) Symbols represent individual 3–5 months old female (orange) and male (blue) WT and J-DTR mice. Data pooled from 3 independent experiments. Horizontal lines represent mean ± SEM. WT PBS: female n = 1, male n = 2; WT DT: female n = 2, male n = 2; J-DTR PBS: female n = 3, male n = 2; J-DTR DT: female n = 3, male n = 3. Statistics: Unpaired Student’s t test with comparisons made between PBS and DT treatments within a genotype (related to ).

Article Snippet: Well backings were removed, and plates dried at RT O/N after which “spots” were visualized with a Mabtech ASTOR ELISPOT reader.

Techniques: Enzyme-linked Immunospot, Concentration Assay